Journal: Pharmaceutical Biology

Article Title: Esculin protects against methionine choline-deficient diet-induced non-alcoholic steatohepatitis by regulating the Sirt1/NF- κ B p65 pathway

doi: 10.1080/13880209.2021.1945112

Figure Lengend Snippet: Esculin attenuates MCD diet-induced fibrosis and inflammation. (A) Representative Masson trichrome staining images of the liver section from each group (400× magnification, scale bar: 50 µm). (B) Representative images from the immunohistochemistry analysis of the collagen 4A1 protein in the liver of each group (400× magnification, scale bar: 50 µm). (C) Representative immunohistochemical images of the fibronectin protein in the liver of each group (400× magnification, scale bar: 50 µm). (D) Representative immunohistochemical images of the F4/80 protein in the liver of each group (400× magnification, scale bar: 50 µm). (E) Level of TNF-α in the liver tissue. (F) Level of IL-6 in the liver tissue. (G) Level of IL-1β in the liver tissue. Data are expressed as mean ± SEM, n = 3. *** p < 0.001 vs. MCS group. # p < 0.05, ## p < 0.01, ### p < 0.001 vs. MCD group. MCS: methionine and choline supplemented; MCD: methionine and choline-deficient.

Article Snippet: The anti-fibronectin, anti-collagen 4A1, and anti-β-actin antibodies, and the TNF-α, IL-6, and IL-1β ELISA kits were purchased from Boster Biological Technology, Ltd. (Wuhan, China).

Techniques: Staining, Immunohistochemistry, Immunohistochemical staining

Journal: Pharmaceutical Biology

Article Title: Esculin protects against methionine choline-deficient diet-induced non-alcoholic steatohepatitis by regulating the Sirt1/NF- κ B p65 pathway

doi: 10.1080/13880209.2021.1945112

Figure Lengend Snippet: Sirt1 silencing affects the activity of esculin on FFA-induced inflammation and fibrosis. (A) Western blot analysis of NF-κB p65, ac-NF-κB p65, fibronectin, collagen 4A1, and β-actin. (B) Densitometric analyses of NF-κB p65. (C) Densitometric analyses of ac-NF-κB p65. (D) Densitometric analyses of fibronectin. (E) Densitometric analyses of collagen 4A1. (F) Relative NADPH/NADP + ratio. Data are expressed as mean ± SEM, n = 3. ** p < 0.01, *** p < 0.001 vs. control group. # p < 0.05, ## p < 0.01 vs. FFA group. $ p < 0.05 vs. FFA + esculin + Si-NC group. FFA: free fatty acid, NC: negative control.

Article Snippet: The anti-fibronectin, anti-collagen 4A1, and anti-β-actin antibodies, and the TNF-α, IL-6, and IL-1β ELISA kits were purchased from Boster Biological Technology, Ltd. (Wuhan, China).

Techniques: Activity Assay, Western Blot, Control, Negative Control

Journal: Stem Cells and Development

Article Title: Antiphotoaging Effect of Conditioned Medium of Dedifferentiated Adipocytes on Skin In Vivo and In Vitro: A Mechanistic Study

doi: 10.1089/scd.2014.0321

Figure Lengend Snippet: Subcutaneous injection of concentrated DA-CM increases the collagen types I and III expression in photoaged mouse dermis. Skin punch biopsies were taken at indicated days post injection of concentrated DA-CM in the mouse skin. Immunohistological staining for collagen type I (A) and collagen type III (B) was measured. The pictures were taken under ×200 magnification. Quantification of the protein levels of collagen type I (C) and collagen type III (D) was measured by ImageJ. Data are mean±SD, n=8, *P<0.05 versus injection of same volume of concentrated DMEM as a control. Color images available online at www.liebertpub.com/scd

Article Snippet: The primary antibodies were used against collagen type I (Santa Cruz Biotechnology) and collagen type III (Boster), MMP-1(Boster), and MMP-3 (Boster).

Techniques: Injection, Expressing, Staining, Control

Journal: Stem Cells and Development

Article Title: Antiphotoaging Effect of Conditioned Medium of Dedifferentiated Adipocytes on Skin In Vivo and In Vitro: A Mechanistic Study

doi: 10.1089/scd.2014.0321

Figure Lengend Snippet: DA-CM prevents UVB effects on the alteration of collagen I, collagen III, MMP-1, and MMP-3 proteins in HDFs. HDFs were cultured in DA-CM and exposed to sham or subcytotoxic UV irradiation. The medium were collected at indicated times post UV irradiation. The protein levels of collagen type I (A), collagen type III (B), MMP-1 (C), and MMP-3 (D) were measured by ELISA. Data are mean±SD, n=3, *P<0.05 for HDF+UV versus HDF and HDF+UV+DA-CM versus HDF+UV.

Article Snippet: The primary antibodies were used against collagen type I (Santa Cruz Biotechnology) and collagen type III (Boster), MMP-1(Boster), and MMP-3 (Boster).

Techniques: Cell Culture, Irradiation, Enzyme-linked Immunosorbent Assay

Journal: Stem Cells and Development

Article Title: Antiphotoaging Effect of Conditioned Medium of Dedifferentiated Adipocytes on Skin In Vivo and In Vitro: A Mechanistic Study

doi: 10.1089/scd.2014.0321

Figure Lengend Snippet: TGF-β1 in DA-CM plays a major role in preventing against the UV effects on collagen and MMPs expression. HDFs were cultured in DA-CM and treated with TGF-β1-neutralizing antibody (0.2 μg/mL). At indicated times post sham or UVB irradiation, the cultured medium was collected and the protein levels of collagen type I (A), collagen type III (B), MMP-1 (C), and MMP-3 (D) were measured by ELISA. Data are mean±SD, n=3, *P<0.05 for HDF+UV+DA-CM versus HDF+UV+DA-CM+anti-TGF-β1.

Article Snippet: The primary antibodies were used against collagen type I (Santa Cruz Biotechnology) and collagen type III (Boster), MMP-1(Boster), and MMP-3 (Boster).

Techniques: Expressing, Cell Culture, Irradiation, Enzyme-linked Immunosorbent Assay